• Application Note

Analysis of Caffeine and Metabolites Using XBridge C18

Analysis of Caffeine and Metabolites Using XBridge C18
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of caffeine and metabolites using XBridge C18 Columns.

Introduction

Experimental

Test Conditions

Column:

XBridge C18, 5 μm, 4.6 x 150 mm p/n: 186003116

Mobile Phase A:

5 Mm ammonium acetate pH 7.5

Mobile Phase B:

acetonitrile

Flow Rate:

1.0 mL/min

Injection Volume:

10 μL

Sample Concentration:

20 μg/mL in water

Temperature:

25 ˚C

Detection:

UV @ 273 nm

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time (min)

%A

%B

0.0

98

2

15.0

80

20

18.0

80

20

19.0

98

2

25.0

98

2

Results and Discussion

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WA60169, April 2007

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