This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the separation of Catechin mixture found in tea using SunFire IS Column.
Compounds use in this study:
1. Caffeine
2. Epicatechin
3. Epigallocatechin Gallate
Column: |
SunFire C8 4.6 x 150 mm, 5 μm |
Part number: |
186002549 |
Mobile phase A: |
0.1% HCOOH in Water |
Mobile phase B: |
0.1% HCOOH in Acetonitrile |
Flow rate: |
3 mL/min |
Injection volume: |
10 μL |
Sample concentration: |
20 μg/mL in water |
Temperature: |
30 °C |
Detection: |
UV @ 280 nm |
Instrument: |
Alliance 2695 with 2996 PDA |
WA41888, March 2005