• Application Note

Cytochrome c Tryptic Digest - Column Lifetime Study, Critical Pair Resolution

Cytochrome c Tryptic Digest - Column Lifetime Study, Critical Pair Resolution
  • Waters Corporation
Download PDF Download PDF
Cytochrome molecule structure

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of cytochrome c tryptic digest.

Introduction

The compound analyzed in this study is cytochrome c tryptic digest.

Experimental

HPLC Method

Column:

Symmetry 300 C18, 3.9 x 150 mm, 5 μm

Part Number:

WAT106154

Mobile phase A:

0.1% TFA water/acetonitrile 90:10

Mobile phase B:

0.1% TFA water/acetonitrile 40:60

Flow rate:

0.75 mL/min

Injection Volume:

20 μL

Temperature:

35 °C

Detection:

UV @ 214 nm

Gradient

Gradient.

Results and Discussion

Featured Products

WA31763.66, June 2003

Back To Top Back To Top