• Application Note

Atrazine and Its Metabolites in Drinking Water

Atrazine and Its Metabolites in Drinking Water
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of atrazine and its metabolites in drinking water. 

Introduction

The compounds used in this study are –  

  1. Hydroxydesisopropylatrazine 
  2. Desethyldesisopropylatrazine  
  3. Hydroxydesethylatrazine  
  4. Desisopropylatrazine  
  5. Hydroxyatrazine  
  6. Desethylatrazine  
  7. Atrazine

Experimental

HPLC Method

Column:

Symmetry Shield RP18, 3.9 x 150 mm, 5 µm

Part number:

186000108

Mobile phase A:

20 mM Phosphate buffer, pH 6.8

Mobile phase B:

Acetonitrile

Flow rate:

0.8 mL/min

Injection volume:

80 µL

Detection:

PDA (215 nm)

Gradient

Gradient

Results and Discussion

Drinking water samples (100 mL) were spiked with the herbicides and adjusted to pH 1.5. The samples were then analyzed using 6 cc Oasis MCX cartridges using the protocol for basic compounds. 

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WA31764.47, June 2003

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