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Propranolol in Rat Plasma by Mixed-Mode SPE and LC-MS/MS (Low Elution Volume)

Propranolol in Rat Plasma by Mixed-Mode SPE and LC-MS/MS (Low Elution Volume)

Blood plasma tubes in two layers divided by centrifuge

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the analysis of Propanolol in Rat Plasma by LC-MS/MS.

Introduction

The compound analyzed in this study is Propanolol.

Propanolol

Experimental

HPLC Method

Column:	XTerra MS C₁₈, 2.1 x 30 mm, 3.5 µm
Part number:	186000398
Mobile phase A:	Water + 0.5 % NH₄OH
Mobile phase B:	ACN + 0.5 % NH₄OH
Flow rate:	0.2 mL/min
Temperature:	Ambient
LC instrument:	Alliance 2795

Gradient

Time (min)	Profile
	%A	%B
0	5	95
1	95	5

MS Conditions

MS instrument:	Micromass Quattro Triple Quadrupole
Ion source:	ESI+
Source temp.:	150 °C
Gas cell:	2.0 e^-3 bar Argon
Desolvation temp.:	350 ˚C
Drying gas flow:	500 L/hr
Cone gas flow:	50 L/hr
Cone voltage:	25 V
Collision energy:	20
Capillary voltage:	3.5 Kv
MRM transition:	Metoclopramide (IS) m/z 299.8 → 226.7 Propranolol m/z 259.9 →154.9 Amitriptyline m/z 278.1 →232.9 Nortriptyline m/z 263.9 →190.8

Oasis plate

Extraction Method

Results and Discussion

Caliberation Curve

Results

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WA31764.132, June 2003