• Application Note

Phencyclidine in Human Urine

Phencyclidine in Human Urine
  • Waters Corporation
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Urine sample cup on table with test strips

Abstract

This application brief highlights the analysis of phencyclidine in human urine using SymmetryShield RP18.

Introduction

Phencyclidine has been determined in human urine in this application brief.

Experimental

HPLC Method

Column:

SymmetryShield RP18, 3.9 x 150 mm, 5 μm (p/n: 186000108)

Guard Column:

Sentry guard column RP18 3.9 x 20 mm, 5 μm (p/n: 186000107)

Mobile phase:

50 mM Phosphate buffer, pH 7/Acetonitrile 70:30

Flow rate:

1.0 mL/min

Injection volume:

20 μL urine extract

Temperature:

30° C

Detection:

UV @ 210 nm (0.01 AUFS)

Results and Discussion

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WA31763.124, June 2003

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