Flavonoids in Ginkgo - Commercial Products and Whole Leaf

This application brief highlights the analysis of flavonoids in Ginkgo

The compounds analyzed in this application brief are quercetin, kaempferol, and isorhamnetin

Sample Pre-Preparation

1 g sample is refluxed in 50 mL of ethanol: 3M HCl (70:30) for 2.5 hr. The cooled sample is adjusted to exactly 100 mL 0.3 mL of the ethanolic extract is diluted 1:10 with water.

• Recovery, measured with certified standards at 100 ppm in reagent water, was 82% for quercitin and >90 % for the other compounds
• All analyses gave results within ±40 % of the expected values with the exception of the capsule (+60%)
• The selective SPE extraction and cleanup procedure provided a convenient analysis of ginkgo flavonoids in a complex matrix (Herbal One with 16 herbal ingredients)