• Application Note

Diclofenac in Rat Plasma by LC-MS/MS

Diclofenac in Rat Plasma by LC-MS/MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of diclofenac in plasma.

Introduction

Experimental

HPLC Method

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 µm

Part number:

186000398

Mobile phase A:

0.05% HCOOH

Mobile phase B:

ACN

Isocratic mobile phase composition:

60% A; 40% B

Flow rate:

0.2 mL/min

Injection volume:

50 µL

Detection:

MS ESI

Instrument:

Alliance 2790, Micromass Quattro Ultima

Ion source:

ESI

Source temperature:

150 °C

Gas cell:

1.5e-3 mbar, 12 eV

Desolvation temperature:

350 °C

Cone gas flow:

150 L/hr

Drying gas flow:

600 L/hr

Cone voltage:

30 V

Results and Discussion

Featured Products

WA31764.70, June 2003

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