• Application Note

Catechins - 4.6 x 20 mm Intelligent Speed Separation

Catechins - 4.6 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of catechins using 4.6 x 20 mm intelligent speed separation Columns.

Introduction

The compounds used in this study are –

  1. Caffeine
  2. Epicatechin
  3. Epigallocatechin Gallate

Experimental

HPLC Method

Column:

Symmetry C18, 4.6 x 20 mm, 3.5 μm IS

Symmetry Shield RP18, 4.6 x 20 mm, 3.5 μm IS

Part numbers:

Symmetry - 186002090, Shield - 186002092

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in MeOH

Flow rate:

3.0 mL/min

Injection volume:

10 μL

Temperature:

30 °C

Detection:

UV @ 280 nm

Gradient

Gradient.

Results and Discussion

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WA31763.48, June 2003

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