This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of acetaminophen and hydrocodone bitartrate using Symmetry C18 Columns.
Coumpounds used in this study are:
1. Acetaminophen
2. Hydrocodone Bitartrate
            
        | 
                     Column:  | 
                     Symmetry C18, 3.9 x 150 mm, 5 μm (p/n: WAT046980)  | 
            
| 
                     Mobile phase:  | 
                     50 mM Ammonium phosphate, pH 5.8/acetonitrile 85:15  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     20 μL of 775 μg/mL acetaminophen and 8 μg hydrocodone bitartrate sample  | 
            
| 
                     Detection:  | 
                     UV @ 214 nm  | 
            
            
        WA31763.12, June 2003