 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of ephedra alkaloids.
The compounds used in this study are –
 
            
        | Column: | Xterra Phenyl, 2.1 x 150 mm, 3.5 μm | 
| Part number: | 186001181 | 
| Mobile phase A: | 10 mM NH4HCO3, pH 9.5 | 
| Mobile phase B: | ACN | 
| Flow rate: | 0.23 mL/min | 
| Isocratic mobile phase composition: | 90% A; 10% B | 
| Injection volume: | 5 μL | 
| Temperature: | 40 °C | 
| Detection: | UV @ 254 nm | 
| Instrument: | Alliance 2695, 2996 PDA | 
 
            
        WA20738.048, June 2002