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Antidepressants in TFA

Antidepressants in TFA

  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of antidepressants in TFA.

Introduction

The compounds used in this study are - 

  1. Doxepin
  2. Desipramine
  3. Trimipramine

Experimental

SunFire Column mass loading comparison for basic compounds (tricyclic antidepressants) in TFA. Comparison made with other leading HPLC column brands using 3,000 μg total load.

Conditions

Column:

SunFire C18 4.6 x 150 mm, 5 μm

Part number:

186002559

Mobile phase A:

0.1% TFA in Water

Mobile phase B:

0.1% TFA in ACN

Flow rate:

1.4 mL/min

Injection volume:

10 μL

Sample concentration:

100 mg/mL each, in DMSO

Temperature:

Ambient

Detection:

UV @ 254 nm

Instrument:

AutoPurification System

Results and Discussion

Featured Products

WA41905, May 2005

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