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Salicylic Acid and Related Compounds in Serum

Salicylic Acid and Related Compounds in Serum

  • Waters Corporation
Skin lightening cream product application on face

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis salicylic acid and related compounds in serum.

Introduction

The compounds used in this study are –  

  1. Benzoic acid
  2. Nalidixic acid (I.S.)
  3. Salicylic acid

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 3.0 x 150 mm, 5 μm

Guard column:

Sentry Guard Column, 3.9 x 20 mm, 5 μm

Part numbers:

Column - WAT094243, Guard - WAT200675

Mobile phase:

1.2% glacial acetic acid/acetonitrile 75:25

Flow rate:

0.6 mL/min

Injection volume:

10 μL of reconstituted porcine serum extract

Temperature:

40 °C

Detection:

UV @ 236 nm

Results and Discussion

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample.

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WA31763.145, June 2003

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