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Propranolol in Serum

Propranolol in Serum

  • Waters Corporation
Plasma preparation for centrifuge

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of propranolol in serum using SymmetryShield Columns. 

Introduction

The compounds analyzed in this study are:

  1. Propranolol 
  2. Butyl paraben (I.S.)
Propranolol
Butyl Paraben

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 3.9 x 150 mm, 5 μm

Guard column:

Symmetry guard column, 3.9 x 20 mm, 5 μm

Part numbers:

Column - WAT200655, Guard - WAT200675

Mobile phase:

20 mM potassium phosphate, pH 7/methanol 38:62

Flow rate:

1.0 mL/min

Injection volume:

20 μL of reconstituted porcine serum extract

Temperature:

30 °C

Detection:

UV @ 254 nm

Extraction Method

Results and Discussion

Recovery and %RSD

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

Chromatogram of Serum Extracts

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WA31763.135, June 2003

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