This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of flunitrazepam.
The compound analyzed in this study is flunitrazepam (USP Tailing Factors - 1.2).
            
        | 
                     Column:  | 
                     Symmetry C18, 3.9 x 150 mm, 5 μm  | 
            
| 
                     Part number:  | 
                     WAT046980  | 
            
| 
                     Mobile phase:  | 
                     50 mM potassium phosphate pH 6.0/acetonitrile 60:40  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     10 μL of 30 μg/mL flunitrazepam  | 
            
| 
                     Detection:  | 
                     UV @ 219 nm  | 
            
            
        WA31763.85, June 2003