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BSA Tryptic Digest - Column Length Effects

BSA Tryptic Digest - Column Length Effects
  • Waters Corporation
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Bovine serum albumin (BSA) molecular structure

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of BSA tryptic digest.

Introduction

The compound analysed in this study is BSA Tryptic Digest.

Experimental

HPLC Method

Column A:

Symmetry 300 C18, 4.6 x 50 mm, 5 μm

Column B:

Symmetry 300 C18, 4.6 x 150 mm, 5 μm

Column C:

Symmetry 300 C18, 4.6 x 250 mm, 5 μm

Part number:

A: WAT106209

B: WAT106157

C: WAT106151

Mobile phase A:

0.1% TFA in water

Mobile phase B:

0.1% TFA in acetonitrile

Flow rate:

0.75 mL/min

Injection volume:

20 μL (7 μL for 4.6 x 50 mm)

Temperature:

35 °C

Detection:

UV @ 214 nm

Gradient

Gradient.

Results and Discussion

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WA31763.40, June 2003

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