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Antiepileptics and Metabolites

Antiepileptics and Metabolites
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of antiepileptics and its metabolites using Symmetry Columns. 

Introduction

The compounds analyzed in this study are:

  1. 2-Phenyl-2ethylmalonamide 
  2. Phenobarbital  
  3. p-Hydroxyphenobarbital 
  4. Carbamezapine-10,11-epoxide 
  5. Primidone 
  6. Carbamezapine 
  7. 10,11-Dihydrocarbamezapine 
  8. Phenytoin 

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 µm

Guard column:

Symmetry Guard Column 3.9 x 20 mm, 5 µm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase:

100 mM potassium phosphate, pH 6.9/acetonitrile/methanol/water 17:25:5:53

Flow rate:

1.0 mL/min

Injection volume:

5 µL of 25µg/mL sample

Detection:

UV @ 214 nm

USP Tailing Factors

  1. NA
  2. NA
  3. 1.13
  4. 1.17
  5. 1.10
  6. 1.17
  7. NA
  8. NA

Results and Discussion

Featured Products

WA31763.30, June 2003

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