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QC of Synthetic Oligonucleotides

QC of Synthetic Oligonucleotides
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates QC of synthetic oligonucleotides.

Introduction

The compound analyzed in this study is synthetic oligonucleotides.

Experimental

Conditions

Column:

Xterra MS C18 4.6 x 50 mm, 2.5 μm

Part number:

186000602

Mobile phase A:

5% ACN, 95% 100 mM Triethylammonium acetate, pH 7.0

Mobile phase B:

20% ACN, 80% 100 mM Triethylammonium acetate, pH 7.0

Flow rate:

1.0 mL/min

Injection volume:

0.6 μL

Temperature:

60 °C

Detection:

UV @ 260 nm

Instrument:

Alliance 2695, 2996 PDA

Gradient

Time(min)

Profile

%A

%B

0.0

90.7

9.3

20.8

56.0

44.0

Results and Discussion

Featured Products

WA20738.095, June 2002

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