Multi-Residue Analysis of Pesticides in Rolled Oats Using AOAC QuEChERS Method by UPLC-MS/MS

This application brief higlights Multi-Residue Analysis of Pesticides in Rolled Oats.

Extraction Procedure

1. Add 15 mL 1% acetic acid in acetonitrile into the 50 mL DisQuE extraction tube 1.
   
2. Diluted 7.5 g ground rolled oats with 15 mL water and soak for 10 min.
3. Add sample into the 50 mL tube.
4. Add any internal standards and standard mixture.
5. Shake vigorously for 1 minute and centrifuge > 1500 rcf for 5 minute.
6. Transfer 1 mL of the acetonitrile extract into the clean-up tube 2.
7. Shake for 30 seconds and centrifuge >1500 rcf for 1 minute.
8. Transfer 100 μL of final extract into a 1.5 mL centrifuge tube.
9. Add any post-extraction internal standards.
10. Dilute as needed with an appropriate buffer or solvent.
11. Centrifuge > 16000 rcf for 5 minutes.
12. Transfer to autosampler vial.