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Spiramycin in Pork

Spiramycin in Pork
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This method can be used to monitor Spiramycin, a macrolide antibiotic, in pork.

Introduction

World health organizations are concerned about the antibiotics and antibacterials levels in food. This method can be used to monitor Spiramycin, a macrolide antibiotic, in pork.

Experimental

Pretreatment

  1. Weigh 5 g of sample into 25 mL of water. Homogenize.
  2. Centrifuge at 3000 rpm for 10 minutes.
  3. Collect supernatant.
  4. Re-extract pellet with 15 mL 1.2% metaphosphoric acid/ methanol (50:50, v/v).
  5. Vortex.
  6. Centrifuge at 3000 rpm for 10 minutes. Collect supernatant.
  7. Combine both supernatants.
  8. Filter sample using glass fiber filter.

SPE Procedure

SPE Buffer:

Dissolve 8.7 g dipotassium hydrogen phosphate in 1 L of deionized water, adjust to pH 3 with phosphoric acid.

LC Conditions

System:

ACQUITY UPLC

Column:

ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm

Flow rate:

400 μL/min

Mobile phase A:

0.1% formic acid in water

Mobile phase B:

0.1% formic acid in acetonitrile

Gradient

MS Conditions

MS System:

Waters Quattro Premier XE

MRM transitions:

1: 422.50→101.00

2: 422.50→174.10

Ionization mode:

Positive electrospray (ESI+)

Multiple reaction monitoring

Results and Discussion

Representative chromatogram 100 ppb spiramycin spiked sample in pork muscle. 
Recovery results for 100 ppb spiramycin spiked sample in pork muscle. 

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