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Analysis of Catecholamines Using XBridge Shield RP18 

Analysis of Catecholamines Using XBridge Shield RP18 
  • Waters Corporation
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Tyrosine molecular chemical structure of 4-hydroxyphenylalanine

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of catecholamines using XBridge Shield RP18 Columns.

Introduction

Catecholamines, such as epinephrine (adrenaline), norepinephrine (noradrenaline), and dopamine, are derived from the amino acid tyrosine and act as important hormones or neurotransmitters.

Experimental

Test Conditions

Columns:

XBridge Shield RP18 4.6 X 100 mm, 3.5 μm p/n: 186003044

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM HCOONH4, pH 3.0

Flow rate:

1.0 mL/min

Injection volume:

10 μL

Sample Concentration and Diluent:

10 μg/mL in H2O

Temp.:

30 °C

Sampling Rate:

5 points /second

Detection:

UV @ 280 nm

Time Constant:

1.0

Needle Wash:

5/95 MeOH/H2O

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time(min)

%A

%B

%C

0

90

0

10

5

90

0

10

15

65

25

10

16

65

25

10

17

90

0

10

20

90

0

10

System Suitability Parameters

Results and Discussion

Featured Products

WA60201, June 2007

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