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Naptalam in Cucumber Using LC-MS

Naptalam in Cucumber Using LC-MS
  • Waters Corporation
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Cucumber slices

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of naptalam in cucumber using Oasis SPE products.

Introduction

Naptalam has been studied in this application brief.

Experimental

LC-MS Conditions

Column:

XTerra MS C18, 2.1 x 100 mm

Mobile phase:

25% acetonitrile/75% 10mM ammonium acetate (pH 5.5) to 90% acetonitrile in 6 minutes.

Flow rate:

200 μL/min

Injection volume:

20 μL

LC system:

Waters Alliance Separations Module

MS system:

Waters/Micromass ZMD

Interface:

Positive Electrospray (ESI+)

Multiple Selected-Ion Recording (SIR)

Sample Preparation

  • Prepare 8 gm sample (homogenize)
  • Extract with 30 mL acetonitrile (shake 30 min)
  • Centrifuge (10 min @ 8000 x g)
  • Transfer supernatant to reservoir
  • Wash pellet with 20 mL water
  • Transfer wash to reservoir (combine with supernatant)
  • Perform SPE

Results and Discussion

Featured Products

WA31764.109, June 2003

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