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Nalidixic Acid Antibiotics, Isocratic – 4.6 x 20 mm Intelligent Speed Separation

Nalidixic Acid Antibiotics, Isocratic – 4.6 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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Red and white antibiotic capsules

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of nalidixic acid antibiotics.

Introduction

The compounds used in this study are –

  1. Cinoxacin
  2. Oxolinic Acid
  3. Nalidixic Acid

Experimental

Conditions

Column:

Atlantis dC18, 4.6 x 20 mm IS, 3 μm, (p/n: 186002062)

Mobile phase A:

Water

Mobile phase B:

Methanol

Mobile phase C:

Acetonitrile

Mobile phase D:

1% HCOOH, pH 2.23

Flow rate:

4 mL/min

Isocratic mobile-phase composition:

55% A, 30% B, 5% C, 10% D

Injection volume:

5 μL

Sample concentration:

5 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Waters Alliance 2965 Separations Module with Waters 2996 PDA

Results and Discussion

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WA31787.14, June 2003

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