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Nalidixic Acid Antibiotics by LC-MS, 5.0 Minute Gradient – 2.1 x 20 mm Intelligent Speed Separation

Nalidixic Acid Antibiotics by LC-MS, 5.0 Minute Gradient – 2.1 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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Opened amoxicillin capsule with powder

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on the analysis of nalidixic acid antibiotics by LC-MS. 

Introduction

The compounds analyzed in this study are:

Compounds
Compounds

Experimental

LC Conditions

Column:

Atlantis dC18, 2.1 x 20 mm IS, 3.0 μm, (P/N: 186002058)

Mobile phase A:

Water

Mobile phase B:

Methanol

Mobile phase C:

1% HCOOH in Water

Flow rate:

0.4 mL/min

Injection volume:

2 μL

Sample concentration:

10 μg/mL

Temperature:

30°C

Instrument:

Alliance 2795 and Waters ZQ

Gradient

Gradient

MS Conditions

Waters ZQ

ES+

Capillary (kV):

3.5

Cone (V):

5.0

Extractor:

3.0

RF lens:

0.1

Source temp. (°C):

150

Desolvation temp. (°C):

400

Cone gas flow (L/Hr):

50

Desolvation gas fLow(L/Hr):

500

LM resolution:

15

HM resolution:

15

Ion energy:

0.5

Multiplier (V):

650

Results and Discussion

The top figure is the total ion current, followed by the extracted ion signals for each of the three analytes. 

*The “extra” peak in the cinoxacin panel is the isotope from oxolinic acid. 

Results

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