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Endocrine Disruptors in Soil

Endocrine Disruptors in Soil
  • Waters Corporation
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Farmer holding pile of arable soil in hands

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of endocrine disruptors in soil.

Introduction

The compounds used in this study are –

  1. Benomyl
  2. Carbaryl
  3. Atrazine
  4. Bisphenol A

Experimental

HPLC Method

Column:

Symmetry C18, 3.9 x 150 mm, 5 μm

Part number:

WAT046970

Mobile phase A:

10 mM phosphate pH 6.8

Mobile phase B:

Methanol

Flow rate:

1.0 mL/min

Injection volume:

100 μL

Sample:

10 g potting soil extracted with 25 mL acetonitrile; then SPE on Oasis HLB

Detection:

PDA (225 nm extracted, 0.04 AUFS)

Gradient

Gradient.

Soil samples (5 g) were spiked with the appropriate compounds and extracted with 25 mL of acetonitrile (30 minutes on shaker). A 5 mL aliquot of the acetonitrile extract was diluted to 100 mL with reagent water (MilliQ) and then processed by SPE.

Results and Discussion

Featured Products

WA31763.81, June 2003

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