Advil Allergy Sinus Tablet: Oasis MCX

Condition:

500 μL MeOH

Equilibrate:

500 μL H₂O

Load:

500 μL sample (250 μL plasma diluted 1:1 with 4% H₃PO₄ in H₂O)

Wash 1:

500 μL 2% FA

Elute 1:

2 x 125 μL MeOH ( Ibuprofen)

Elute 2:

2 x 125 μL 5% NH₄OH in MeOH (Bases)

Options:

1. Dilute Elute 2 with 250 μL 2% FA in water and Elute 1 with 250 μL 100% water and analyze separately.

2. Combine the two elutions and evaporate/reconstitute.

Pseudoephedrine HCl (Base):

1.5 μg/mL

Chlorpheniramine Maleate (Base):

0.1 μg/mL

Ibuprofen (Acid):

10 μg/mL

Column:

ACQUITY UPLC BEH C₁₈, 2.1 x 50 mm, 1.7 μm

Mobile phase A:

0.1% HCOOH in H₂O

Mobile phase B:

0.1% HCOOH in MeOH

Flow rate:

0.3 mL/min

Injection volume:

10.0 μL

Column temp:

4 °C

Sample temp:

10 °C

Instrument:

ACQUITY UPLC with Quattro Premier