• Application Note

Gradient Separation of Morphine and Morphine-3-β-Glucuronide on ACQUITY UPLC BEH HILIC

Gradient Separation of Morphine and Morphine-3-β-Glucuronide on ACQUITY UPLC BEH HILIC
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates gradient separation of morphine and morphine-3-β-glucuronide on ACQUITY UPLC BEH HILIC Columns.

Introduction

The compounds used in this study are:

  1. Morphine
  2. Morphine-3-β-Glucuronide

Experimental

Test Conditions

Column:

ACQUITY UPLC BEH HILIC, 2.1 x 100 mm, 1.7 μm

Part Number:

186003461

Mobile Phase A:

10 mM NH4COOH, 0.2% HCOOH in 50:50 ACN:H2O

Mobile Phase B:

10 mM NH4COOH, 0.2% HCOOH in 90:10 ACN:H2O

Flow Rate:

0.788 mL/min

Injection Volume:

2.1 μL

Sample Concentration:

125 μg/mL

Sample Diluent:

75:25 ACN:MeOH with 0.2% HCOOH

Temperature:

30 °C

Detection:

UV @ 280 nm

Sampling Rate:

20 pts/sec

Time Constant:

0.1

Instrument:

Waters ACQUITY UPLC with ACQUITY TUV

Gradient:

Time (min)

Profile

%A

%B

0.00

0.1

99.9

0.37

0.1

99.9

1.46

99.9

0.1

1.50

0.1

99.9

2.00

0.1

99.9

Results and Discussion

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WA60136, August 2009

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