This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of sulfa drugs in serum.
The compounds used in this study are –
            
        | 
                     Column:  | 
                     Symmetry C18, 3.9 x 150 mm, 5 μm  | 
            
| 
                     Part number:  | 
                     WAT046980  | 
            
| 
                     Mobile phase:  | 
                     1% glacial acetic acid and 4% methanol in water  | 
            
| 
                     Flow rate:  | 
                     1 mL/min  | 
            
| 
                     Injection volume:  | 
                     10 μL of reconstituted porcine serum extract  | 
            
| 
                     Temperature:  | 
                     25 °C  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
            
        
            
        
            
        WA31764.142, June 2003