Phenolics in Echinacea

This application brief highlights the analysis of phenolics in echinacea using Symmetry columns.

Phenolic compounds are analysed in complex herbal tea extracts using Symmetry columns.

Sample Preparation

100 mg sample is extracted with 25 mL of ethanol:water (70:30)

1 mL of the ethanolic extract is diluted 1:3 with water (~ pH 7)

• Recovery, measured with certified standards at 100 ppm in reagent water, was greater than 85 % for all compounds
  
• All analyses gave results within ± 35 % of the expected values with the exception of the liquid supplement (see goldenseal analysis)
  
• The selective SPE extraction and cleanup procedure provided a convenient analysis of echinacea phenolics in complex matrix such as in herbal tea