• Application Note

Caffeine and Metabolites in Serum

Caffeine and Metabolites in Serum
  • Waters Corporation
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Platelet-rich plasma vials in rack

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of caffeine and metabolites in serum.

Introduction

The compounds used in this study are –

  1. Theobromine
  2. Paraxanthine
  3. Theophylline
  4. β-hydroxyethyltheophylline (I.S.)
  5. Caffeine

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 3.0 x 150 mm, 5 μm

Guard column:

Sentry Guard Column, 3.9 x 20 mm, 5 μm

Part number:

Column - WAT094243, Guard - WAT200675

Mobile phase:

20 mM ammonium acetate, pH 5/acetonitrile, 95:5

Flow rate:

0.6 mL/min

Injection volume:

20 μL of reconstituted porcine serum extract

Temperature:

25 °C

Detection:

UV @ 273 nm

Results and Discussion

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

Chromatogram of Serum Extracts: A) Blank B) Spiked Sample

Featured Products

WA31763.45, June 2003

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