• Application Note

Diphenhydramine - pH 9.5, LC-MS

Diphenhydramine - pH 9.5, LC-MS
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of diphenhydramine by LC-MS.

Introduction

The compound analyzed in this study is diphenhydramine.

Experimental

Conditions

Column:

Xterra MS C18 2.1 x 30 mm, 3.5 μm

Part number:

186000398

Mobile phase A:

0.1% NH4OH in H2O

Mobile phase B:

0.1% NH4OH in ACN

Flow rate:

0.2 mL/min to MS

Isocratic mobile phase composition:

55% A; 45% B

Injection volume:

20 μL of 100 pg/μL

Temperature:

Ambient

Detection:

MS ESI+, SIR 256.12

Instrument:

Alliance 2795 HT, Micromass ZQ

MS Conditions

Micromass ZQ ESI+

Capillary (KV):

3.0

Cone (V):

15

Extractor:

3.0

RF lens:

0.5

Source temp.:

150

Desolvation temp.:

350

Cone gas flow (L/Hr):

60

Desolvation gas flow (L/Hr):

500

LM resolution:

15

HM resolution:

15

Ion energy:

1.0

Multiplier (V):

650

Results and Discussion

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