• Application Note

Diphenhydramine - LC/UV

Diphenhydramine - LC/UV
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of diphenhydramine using XTerra RP18 columns.

Introduction

Diphenhydramine has been analyzed using LC/UV in this study.

Experimental

HPLC Conditions

Column:

XTerra RP18 4.6 x 150 mm, 5 μm (p/n: 186000492)

Mobile phase:

At pH 3.0: H2O/ACN/100 mM, NH4COOH, pH 3.0, 40: 50: 10

At pH 7.0: H2O/ACN/100 mM, NH4HCO3, pH 7.0, 50: 40: 10

At pH 10.0 H2O/ACN/100 mM, NH4HCO3, pH 10.0, 20: 70: 10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 250 μg/mL

Temperature:

30 °C

Detection:

UV @ 260 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile Phase pH

USP Tailing

3.0

1.25

7.0

1.70

10.0

1.18

 

Results and Discussion

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WA20738.038, June 2002

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