• Application Note

Diclofenac in Rat Plasma

Diclofenac in Rat Plasma
  • Waters Corporation
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Platelet-rich plasma vials in rack

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of diclofenac using XTerra MS C18 columns.

Introduction

Diclofenac in rat plasma has been analyzed in this study.

Experimental

HPLC Conditions

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 μm (p/n: 186000398)

Mobile phase A:

0.05% HCOOH

Mobile phase B:

ACN

Isocratic mobile phase composition:

60% A; 40% B

Flow rate:

0.2 mL/min

Injection volume:

50 μL

Detection:

MS ESI-

Instrument:

Alliance 2790, Micromass Quattro Ultima

MS Conditions

Ion source:

ESI-

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar, 12 eV

Desolvation temp.:

350 °C

Cone gas flow:

150 L/hr

Drying gas flow:

600 L/hr

Cone voltage:

30 V

Results and Discussion

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WA20738.037, June 2002

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