Multi-Residue Analysis of Pesticides in Avocado Using AOAC QuEChERS Method by UPLC-MS/MS

This application brief demonstrates multi-residue analysis of pesticides in avocado using AOAC QuEChERS method by UPLC-MS/MS.

Extraction Procedure

1. Add 15 mL 1% acetic acid in acetonitrile into the 50 mL DisQuE extraction tube.
2. Add 15 g of homogenized sample into the 50 mL tube.
3. Add any internal standards and standard mixture.
4. Shake vigorously for 1 minute and centrifuge > 1500 rcf for 5 minute.
5. Transfer 1 mL of the acetonitrile extract into the 2 mL clean-up tube containing 50 mg PSA, 150 mg MgSO₄, and 50 mg C₁₈.
6. Shake for 30 seconds and centrifuge >1500 rcf for 1 minute.
7. Transfer 100 μL of final extract into a 1.5 mL centrifuge tube.
8. Add any post-extraction internal standards.
9. Dilute as needed with an appropriate buffer or solvent.
10. Centrifuge > 16000 rcf for 5 minutes.
11. Transfer to autosampler vial.