Analysis of Mono-, Di-, and Oligosaccharides Using ACQUITY UPLC BEH Amide Columns with Acetone as Organic Modifiers

Column:

ACQUITY UPLC BEH Amide 2.1 x 50 mm, 1.7 μm

Part number:

186004800

Mobile phase A:

80/20 acetone/H₂O with 0.05% triethylamine [TEA]

Mobile phase B:

30/70 acetone/H₂O with 0.05% triethylamine [TEA]

Flow rate:

0.17 mL/min

Gradient:

5 minute gradient, 80%–60% MeCN with 10 minute re-equilibration

Injection volume:

0.7 μL (PLNO)

Sample concentration:

1 mg/mL each

Sample diluent:

50/50 MeCN/H₂O

Column temperature:

85 °C

Strong needle wash:

20/80 MeCN/H₂O (800 μL)

Weak needle wash:

75/25 MeCN/H₂O (500 μL)

Seal wash:

50/50 MeCN/H₂O

Instrument:

Waters ACQUITY UPLC with ELSD

Gain:

200

Pressure:

40 psi

Drift tube temperature:

40 °C

Nebulizer:

Cooling

Data rate:

10 pps

Filter time constant:

Normal