This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of steroids in serum.
The compounds used in this study are –
Column: |
Symmetry Shield RP8, 3.9 x 150 mm, 5 μm |
Guard column: |
Sentry Guard Column 3.9 x 20 mm, 5μm |
Part numbers: |
Column - WAT200655, Guard - WAT200675 |
Mobile phase: |
20 mM potassium phosphate, pH 7/methanol 41:59 |
Flow rate: |
1.0 mL/min |
Injection volume: |
5 μL of reconstituted porcine serum extract |
Detection: |
UV @ 254 nm |
WA31763.150, June 2003