 
            
        This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of propranolol in human urine using SymmetryShield columns.
Compounds used in this study are:
1. Metoprolol
2. Oxprenolol (I.S.)
3. Propranolol
 
            
        | Column: | SymmetryShield RP18, 3.9 x 150 mm, 5 μm (p/n: 186000108) | 
| Guard Column: | Sentry guard column RP18 3.9 x 20 mm, 5 μm (p/n: 186000107) | 
| Mobile phase: | 0.1% TFA in water/Acetonitrile 80:20 | 
| Flow rate: | 1.0 mL/min | 
| Injection volume: | 50 μL | 
| Temperature: | 30° C | 
| Detection: | UV @ 275 nm | 
 
            
         
            
         
            
        WA31763.134, June 2003