• Application Note

Pharmaceutical Residues in Environmental Samples – LC-MS And LC/PDA Results of LC/PDA Analysis, 2.5 ppb

Pharmaceutical Residues in Environmental Samples – LC-MS And LC/PDA Results of LC/PDA Analysis, 2.5 ppb
  • Waters Corporation
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Two scientists in protective suits taking water samples from river

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of pharmaceutical residues in environmental samples using optimized Oasis MCX SPE method.

Introduction

Compounds used in this application brief are:
1. Acetaminophen
2. Phenylpropanolamine
3. Salicylic acid
4. Diphenhydramine
5. Clofibric acid
6. Ethynylestradiol
7. Tamoxifen
8. Ibuprofen

Experimental

HPLC Method

Column:

XTerra MS C18 4.6 x 100 mm, 3.5 μm (p/n: 186000436)

Mobile phase A:

15 mM NH4COOH, pH 4.0

Mobile phase B:

MeOH

Flow rate:

1.0 mL/min

Injection volume:

40 μL

Detection:

MS ESI+

Instrument:

Alliance 2695, Micromass ZQ

Gradient

LC-MS Conditions Waters Micromass ZMD

Compound

MW

ESI+

ESI-

Acetaminophen

151.2

152(40) 110(40)

150(20)

Phenylpropanolamine

151.2

152(40) 134(40)

Salicylic acid

138.1

137(17)

Diphenhydramine

255.3

256(25) 167(40)

Clofibric acid

214.7

213(17) 127(17)

Ethynylestradiol

296.4

295(40) 145(80)

Tamoxifen

371.5

372(40)

Ibuprofen

206.3

205(17) 159(30)

Optimized SPE Method For LC-MS Determination of Pharmaceutical Residues in Environmental Samples

Conditions for Oasis MCX 6 cc/150 mg (60 μm) Part Number 186000255

Oasis HLB Plus Part Number 186000132 

Results and Discussion

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WA31764.118, June 2003

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