• Application Note

Theophylline

Theophylline
  • Waters Corporation
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Bronchodilator used for asthma and COPD

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of theophylline.

Introduction

The compound analyzed in this study is theophylline.

Experimental

Conditions

Column:

Xterra RP18 4.6 x 150 mm, 5 μm

Part number:

186000492

Mobile phase:

pH 3.0: H2O/ACN/100 mM NH4COOH, pH 3.0 65:25:10

pH 7.0: H2O/ACN/100 mM NH4HCO3, pH 7.0 85:05:10

pH 10.0 H2O/ACN/100 mM NH4HCO3, pH 10.0 90:0:10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 250 μg/mL

Temperature:

30 °C

Detection:

UV @ 280 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile Phase pH

USP Tailing

3.0

1.1

7.0

1.06

10.0

1.19

Results and Discussion

Featured Products

WA20738.104, June 2002

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