Preparative separations are purposeful and practical, meant for isolating targets from mixtures.
Purification outcomes can be off-target, varying by compound purity, recovery and turnaround times. With Waters solutions, including our Optimum Bed Density (OBD) Preparative Columns and Preparative LC Systems, you can ensure more accurate outcomes for your preparative separations.
Is maximizing target recovery an issue? If peak shape and low sensitivity plague your purification, metal-sensitive compounds may be ‘sticking’ to your stainless-steel column or other metal surfaces in your instrument flow path. Waters MaxPeak High-Performance Surfaces (HPS) Technology can help you achieve predictable scale-up for challenging molecular targets prone to interactions for applications such as organic acids, peptides, and oligonucleotides.
Learn more about preparative solutions for organic acids and peptides
Simple scale-up rules offer practical guidance for purification success on the first try.
Once you’ve learned these five rules, your lab can focus on applying the 5 rules of scale up in LC purification.
Waters OBD Preparative and new Premier OBD Preparative Column Solutions contain robust chromatographic particles designed for the rigors of purification.
With Waters purification solutions, including our highly efficient Preparative Columns, you can directly scale from ultra-performance liquid chromatography (UPLC) and ultra-high performance liquid chromatography (UHPLC) with extended column life and higher recoveries.
Leverage Waters Prep Calculator Tool and Column Coach Tool to help guide you, and support your scale-up from UPLC, UHPLC, or high performance liquid chromatography HPLC to preparative LC.
With the Optimum Bed Density (OBD) design of Waters Preparative HPLC Columns, your lab can ensure consistency in column-to-column performance and increase productivity across your workflows.
Waters proprietary and innovative preparative OBD Technology manufacturing procedure combines hardware design, particle characteristics, and exceptional packed bed densities yielding columns with excellent stability, reproducibility, loadability, and high efficiencies. Waters Preparative Column Wall Chart takes the guesswork out of finding the right column for your lab.
“This is one of the routine columns we used for drug discovery isolation of target compounds. It’s been reliable and easy to manage.”
— Lodo Therapeutics
“The SunFire OBD C18 Prep Column made transitioning from my analytical method to a focused-gradient prep scale method fast and easy. Product gave reproducible results which was critical for impurity isolation and identification.” — BioVectra Inc.
“The separation reproduces well from run to run. The separation of small molecules is great with very sharp peaks.” — Warp Drive Bio
“Waters XBridge Oligonucleotide BEH 300 Å C18 5µm OBD columns showed a definitive improvement in removing early impurities.” – Oligo Factory
Direct scale-up: UPLC to preparative.
Direct scale-up: HPLC to preparative. Resolution maintained at increased loads.
Direct scaleup of the sample mixture on XBridge BEH C18 Column.
Improved lifetime and reproducibility matter. Data from a high-throughput drug discovery laboratory: 7,000 injections on an XBridge BEH C18 OBD Prep Column, 130 Å, 5 µm, 19 x 50 mm. Column longevity varies with operating conditions.
Preparative Chromatography Webinar Series
Join us for our On Demand webinar series. Our comprehensive on-demand webinar series is designed to empower you with practical knowledge and expert insights. Whether you’re a seasoned chromatographer or just starting out, these webinars will enhance your expertise and provide actionable tips for immediate application.
