ACQUITY UPLC I-Class/Xevo TQD IVD System: Analytical Performance for Antibiotics
For in vitro diagnostic use. Not available in all countries.
Introduction
The Waters ACQUITY UPLC I-Class/Xevo TQD IVD System enables the quantification of organic compounds in human biological liquid matrices.
This document describes a test of the analytical performance of the ACQUITY UPLC I-Class/Xevo TQD IVD System for the analysis of ampicillin, azithromycin, cefazolin, cefepime, cefotaxime, ceftazidime, chloramphenicol, ciprofloxacin, clindamycin, daptomycin, flucloxacillin, linezolid, meropenem, piperacillin, sulbactam, and tazobactam in plasma.
Experimental
The ACQUITY UPLC I-Class/Xevo TQD IVD System was controlled by MassLynx Software (v4.2) and the data processed using the TargetLynx XS Application Manager. Calibrators and Quality Controls were prepared by spiking commercially available reference material in plasma and the samples were processed using the following conditions:
Sample Preparation Conditions
A 50 µL sample was processed with methanol and centrifuged, then subsequently diluted with acidified water prior to analysis.
LC Conditions
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Column: |
ACQUITY UPLC BEH C18, 1.7 µm, 2.1 mm x 100 mm |
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Mobile phase A: |
0.1% ammonia in water |
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Mobile phase B: |
Methanol |
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Flow rate: |
0.5 mL/min |
|
Gradient: |
90% A initial, gradient 6 until 0% A at 3.00 minutes, hold until 4.00 minutes, 90% A gradient 6 at 4.10 minutes, then hold until 5.00 minutes |
Gradient
MS Conditions
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Resolution: |
MS1 (0.75 FWHM), MS2 (0.75FWHM) |
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Acquisition mode: |
MRM |
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Polarity: |
ESI (+/-) |
Results and Discussion
Chromatographic selectivity of a range of antibiotics using the ACQUITY UPLC I-Class/ Xevo TQD IVD System is illustrated in Figure 1. Performance characteristics of the antibiotics are shown in Table 1.
*Calibration Range was defined by linear fit where r2 >0.995 for cefepime, daptomycin, piperacillin and sulbactam; for all other analytes a quadratic fit was used. LLOQ defined by S/N (PtP) >10 with %RSD ≤20% and ≤15%. %RSD at LLOQ determined through analytical sensitivity experiments performed over five occasions (n=50). Total precision and repeatability of QCs performed over 5 occasions in plasma (n=25). Data was collected in two runs.
Conclusion
The Waters ACQUITY UPLC I-Class/Xevo TQD IVD System has demonstrated the capability to analyze a panel of antibiotics in plasma.
Disclaimer
The analytical performance data presented here is for illustrative purposes only. Waters does not recommend or suggest analysis of the analytes described herein. These data are intended solely to demonstrate the performance capabilities of the system for analytes representative of those commonly analyzed using liquid chromatography and tandem mass spectrometry. Performance in an individual laboratory may differ due to a number of factors, including laboratory methods, materials used, intra-operator technique, and system conditions. This document does not constitute a warranty of merchantability or fitness for any particular purpose, express or implied, including for the testing of the analytes in this analysis.
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