This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates AQUITY UPLC analysis of 5-Fluorouracil.
Columns: |
ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 μm |
Part Number: |
186004800 |
Isocratic Mobile Phase: |
95/2.5/2.5 MeCN/IPA/H2O with 5 mM CH3COONH4 and 0.02% NH4OH, pH 9.0 |
Flow Rate: |
0.2 mL/min |
Injection Volume: |
5.0 μL (PLNO) |
Sample Concentration: |
50 μg/mL |
Sample Diluent: |
75/25 MeCN/MeOH with 0.2% HCOOH |
Column Temperature: |
25 °C |
Weak Needle Wash: |
95/5 MeCN/H2O |
Detection: |
UV @ 265 nm |
Sampling Rate: |
20 points/sec |
Filter Time Constant: |
0.2 |
Instrument: |
Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector |
WA60092, June 2009