• Application Note

100% Aqueous QC Batch Test on Atlantis T3 Columns

100% Aqueous QC Batch Test on Atlantis T3 Columns
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates 100% aqueous QC batch test on Atlantis T3 Columns.

Introduction

The compounds used in this study are -

  1. Thiourea
  2. 5-Fluorocytosine
  3. Adenine
  4. Guanosine-5’-monophosphate
  5. Thymine

Experimental

Test Conditions

Column:

Atlantis T3, 4.6 x 150 mm, 5 μm

Part number:

186003747

Isocratic mobile phase composition:

10 mM HCOONH4, pH 3.0

Flow rate:

1.2 mL/min

Injection volume:

7 μL

Sample:

Thiourea (35 μg/mL), 5-Fluorocytosine (35 μg/mL), Adenine (15 μg/mL), Guanosine-5’-monophosphate (70 μg/mL), Thymine (45 μg/mL) in 10mM HCOONH4

Column temperature:

Ambient

Sample temperature:

15 ˚C

Detection:

UV @ 254 nm

Sampling rate:

5 point/sec

Filter response:

0.2

Instrument:

Waters Alliance 2695 with 2996 PDA

Results and Discussion

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WA60222, January 2009

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