• Application Note

Analysis of Tricyclic Antidepressants using XBridge Phenyl 

Analysis of Tricyclic Antidepressants using XBridge Phenyl 
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of tricyclic antidepressants using XBridge Phenyl columns.

Introduction

Experimental

Test Conditions

Columns:

XBridge Phenyl, 4.6 x 50 mm, 3.5 μm p/n: 186003332

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4HCO3

Flow rate:

1.2 mL/min

Isocratic Mobile Phase Composition:

48% A; 42% B; 10% C

Injection volume:

10 μL

Sample:

Nordoxepin (10 μg/mL),

Desipramine (10 μg/mL),

Doxepin (10 μg/mL),

Imipramine (10 μg/mL),

Amitriptyline (10 μg/mL) in H2O/ACN (50/50)

Column temp.:

30 °C

Sample temp.:

15 °C

Detection:

UV @ 254 nm

Sampling Rate:

5 points/sec

Filter Response:

0.2

Instrument:

Alliance 2695 with 2996 PDA

Results and Discussion

Compounds
Compounds: 1. Nordoxepin 2. Desipramine 3. Doxepin 4. Imipramine 5. Amitriptyline.

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WA60188, June 2007

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