• Application Note

Analysis of Tricyclic Antidepressants Using XBridge C18, pH 7.0 

Analysis of Tricyclic Antidepressants Using XBridge C18, pH 7.0 
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of tricyclic antidepressants using XBridge C18 Columns at pH 7.0.

Introduction

Tricyclic antidepressants are analyzed in this application brief.

Compounds

Experimental

Test Conditions

Column:

XBridge C18, 5 μm, 4.6 x 150 mm p/n: 186003116

Isocratic Mobile Phase:

20 mM sodium phosphate buffer, pH 7.0/acetonitrile/methanol (30:35:35)

Flow Rate:

1.5 mL/min

Injection Volume:

10 μL

Sample Concentration:

20 μg/mL in water

Temperature:

25 ˚C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695 with 2996 PDA

Results and Discussion

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WA60167, April 2007

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