• Application Note

Sulfonamides – 2.1 x 20 mm Intelligent Speed Separation

Sulfonamides – 2.1 x 20 mm Intelligent Speed Separation
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of Sulfonamides. 

Introduction

The compounds analyzed in this study are: 

  1. Sulfadiazine 
  2. Sulfathiazole  
  3. Sulfamerazine  
  4. Sulfadimidine  
  5. Sulfamethoxazole  
  6. Sulfisoxazole  
  7. Sulfadimethoxine  

Experimental

Conditions

Columns:

Atlantis dC18, 2.1 x 20 mm IS, 3 µm, (P/N:186002058)

Symmetry Shield RP18, 2.1 x 20 mm IS, 3.5 µm, (P/N:186002068)

Symmetry C18, 2.1 x 20 mm IS, 3.5 µm, (P/N:186002066)

XTerra MS C18, 2.1 x 20 mm IS, 3.5 µm, (P/N:186001923)

Mobile phase A:

0.1% HCOOH in Water

Mobile phase B:

0.1% HCOOH in Methanol

Flow rate:

0.6 mL/min

Injection volume:

10 µL

Sample concentration:

20 µg/mL

Temperature:

30 °C

Detection:

UV @ 270 nm

Instrument:

Alliance 2795 with 996 PDA

Results and Discussion

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WA31787.30, June 2003

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