• Application Note

Methadone and Metabolites in Human Urine

Methadone and Metabolites in Human Urine
  • Waters Corporation
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Urine sample in cup on pathology bag

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of methadone and metabiltes in human urine using Oasis MAX extraction method.

Introduction

Compounds studied in this application brief are:

1. EDDP (0.2 μg/mL)

2. Estazolam (I.S.) (0.2 μg/mL)

3. Methadone (0.2 μg/mL)

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 μm (p/n: WAT046970)

Guard Column:

Sentry Guard Column 3.9 x 20 mm, 5 μm (p/n: WAT054250)

Mobile phase:

0.1% TFA in water/Methanol 60:40

Flow rate:

1.0 mL/min

Injection volume:

100 μL urine extract

Temperature:

30 °C

Detection:

UV @ 210 nm

Results and Discussion

Featured Products

WA31764.101, June 2003

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