This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates the analysis of dextromethorphan using Symmetry Columns.
The compound analyzed in this study is dextromethorphan.
            
        | 
                     Column:  | 
                     Symmetry C8, 3.9 x 150 mm, 5 μm  | 
            
| 
                     Guard column:  | 
                     Symmetry Guard Column 3.9 x 20 mm, 5 μm  | 
            
| 
                     Part numbers:  | 
                     Column - WAT046970, Guard - WAT054250  | 
            
| 
                     Mobile phase:  | 
                     23 mM ammonium nitrate, pH 3.4/acetonitrile 30:70  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     20 μL of 100 μg/mL sample  | 
            
| 
                     Detection:  | 
                     UV @ 254 nm  | 
            
            
        WA31763.70, June 2003