• Application Note

Barbiturates in Human Urine

Barbiturates in Human Urine
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of barbiturates in human urine.

Introduction

The compounds used in this study are –

  1. Phenobarbital
  2. Butabarbital
  3. Butalbital
  4. Amobarbital (I.S.)
  5. Mephobarbital
  6. Secobarbital

Experimental

HPLC Method

Column:

Symmetry Shield RP18, 2.1 x 150 mm, 5 μm

Guard column:

Sentry Guard Column RP18, 3.9 x 20 mm, 5 μm

Part number:

Column - 186000111, Guard - 186000107

Mobile phase:

50 mM potassium phosphate, pH 7.0/acetonitrile 71:29

Flow rate:

1.0 mL/min

Injection volume:

80 μL urine extract

Temperature:

30 °C

Detection:

UV @ 214 nm (0.350 AUFS)

Results and Discussion

Chromatogram of A) Blank Urine, B) Spiked Urine

Chromatogram of A) Blank Urine, B) Spiked Urine

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WA31763.34, June 2003

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