• Application Note

Analysis of Ginseng Root Powder on an ACQUITY UPLC BEH Amide Column

Analysis of Ginseng Root Powder on an ACQUITY UPLC BEH Amide Column

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of ginseng root powder on an ACQUITY UPLC BEH Amide column.

Introduction

Compound

Experimental

UPLC Conditions

Column:

ACQUITY UPLC BEH Amide, 1.7 μm, 2.1 x 150 mm

Part Number:

186004802

Mobile Phase:

80:20 MeCN:H2O

Isocratic Flow Rate:

0.6 mL/min

Column Temp.:

60 °C

Sample Temp.:

10 °C

Injection Vol.:

2.5 μL; PLNO on 10 μL loop

Strong & Weak Needle Wash:

95:5 MeCN:H2O

Seal Wash:

10:90 MeOH:H2O

UV:

203 nm

Sampling Rate:

20 Hz

Filter Time Constant:

0.2 sec

Total Run Time:

3.5 min

Instrument:

ACQUITY UPLC with ACQUITY UPLC PDA

Extraction Procedure

  1. Add 1 mL 80% MeOH to 200 mg ginseng root powder and sonicate for 5 min.
  2. Centrifuge for 5 min @ 10,000 rpm.
  3. Keep supernatant and re-extract by repeating steps 2-4 two more times.
  4. Pool all three extracts and mix well.
  5. Filter through a 13 mm nylon 0.2 μm filter prior to injection.

Results and Discussion

WA64083, February 2010

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